- George Jetson (main story and flashback)
- Mr. Spacely (main story and flashback)
- Jane Jetson
- Elroy Jetson
- Tow Truck Guy (only appearance; no lines)
- Judy Jetson
- Mr. Megabucks (only appearance; main story and flashback)
- Spacely Sprocketts (only appearance; no lines; flashback only)
- RUDI (main story and flashback)
- Starla (voice only; only time heard)
- Outer Moongolia
- Orbitty using Bubbles' sad face from The Powerpuff Girls in Hi-Tech Wreck and Haunted Halloween.
- Cosmo: Jetson! You're destroying my career!
George: Sorry, sir. It's R.U.D.I. His system was overloaded.
Cosmo: I hate excuses! Report to Outer Moongolia!
George: I'm going! I'm going!
Cosmo: I'll deal with this goof up computer later!
- George: Take one last look at the old home, everyone! I never thought it'd come to this!
Jane: It's been our only home for so long!
Judy: I grew up here!
Elroy: I'm leaving all my friends!
Elroy, Astro and Orbitty (crying) Boo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo hoo!
Jane: At least we still have each other!
George: And that's important!
Elroy: And after I finally worked out a permanent program for poor R.U.D.I. too!
Astro: (crying) Roo Hoo hoo hoo!
George: Come on, let's go! Don't look back! It's time to move on to our new life on Outer Moongolia!
Elroy and Judy: Yuck!